Notch信號靶標PCR芯片可用于研究Notch信號轉導的84個關鍵應答基因的表達。Notch信號通路是一個傳統(tǒng)的發(fā)育途徑，參與細胞間通訊，細胞命運，細胞凋亡和發(fā)育。Notch信號通路首先在果蠅中發(fā)現(xiàn)，哺乳動物同源的Notch信號通路稍晚些被確定。來自Delta及Jagged/Serrate的配體可以激活與膜結合的Notch受體，誘導Notch胞內結構域斷裂。這種胞內結構域與RBPJ家族的轉錄因子以及共激活因子和輔阻遏物的相互作用來啟動靶基因的轉錄。Notch信號通路激活的輸出依賴于細胞內容物，在適當?shù)臅r空調節(jié)激活是正常的胚胎發(fā)育過程所必須的。Notch信號通路失調可能會導致癌癥以及遺傳發(fā)育障礙。通過如ChIP和基因表達研究等實驗技術，很多經(jīng)典的Notch通路靶標基因都已經(jīng)被鑒定出來了。該芯片包含了Notch經(jīng)典信號通路的轉錄因子和研究驗證得到的高關聯(lián)性靶標基因，通過該芯片得到的結果可用于Notch信號傳導激活和抑制研究。通過實時定量PCR的方法，研究者即能夠利用該芯片簡單可靠地同時檢測Notch調節(jié)的細胞活動相關基因的表達。
Cell Fate Commitment: DKK1, HES1, HES5, JAG1, NODAL, NOTCH1, NOTCH3, PAX6, RUNX2, SOX9. Developmental Processes: Embryonic Development: DKK1, EDN1, EFNB1, FOXC1, FOXD3, FOXF1, HES1, HES7, MYF5, NODAL, NOTCH1, PBX1, PCDH8, PDGFRA, PDGFRB, RBPJ, TCF15, VEGFA, WNT4, WNT5A. Muscle Development: EDN1, EGR3, FOXC1, HBEGF (DTR), HEY2, IGFBP3, JAG1, MYF5, NOTCH1, PDGFB, PDGFRB, TNC. Epidermis Development: JAG1, KRT14, NOTCH1, PTGS2 (COX2), SNAI1 (SNAIL), SOX9, TCF15. Immune System Development: ID2, JAG1, JUN, KITLG, PBX1, RUNX1 (AML1), SGPL1, VEGFA. Nervous System Development: BDNF, CCND2, DKK1, DTX1, EFNA1, EFNB1, EGR3, FABP7, FOXC1, HES1, HES5, HEY1, HEY2, HEYL, ID3, ID4, JAG1, KALRN, NES, NODAL, NOTCH1, NOTCH3, PAX6, PBX1, RBPJ, RND1, VEGFA. Vasculature Development: EDN1, EFNA1, FLT1, FOXC1, FOXF1, HEY2, ID1, JAG1, JUN, NODAL, NOTCH1, RBPJ, RUNX1 (AML1), SGPL1, VEGFA. Kidney Development: ADAMTS1, BDNF, FOXC1, PBX1, PDGFRB, SGPL1. Other Development: NRARP, S1PR3. Signal Transduction: Notch Pathway: CREBBP (CBP), DTX1, FOXC1, HES1, HES7, HEY1, HEY2, HEYL, JAG1, LFNG, NOTCH1, NOTCH3, RBPJ, RBPJL, SNW1 (SKIIP). WNT Pathway: CCND1, DKK1, FRZB (FRP-3), FZD5, WISP1, WNT4, WNT5A, WNT6. Apoptosis: ABL1, BDNF, FOXC1, GADD45B, ID3, IGFBP3, JUN, KALRN, NOTCH1, PTCRA, PTGS2 (COX2), SGPL1, SOCS3, SOX9, VEGFA. Cytokines: CX3CL1, CXCL12 (SDF1), CXCL2, IL33, NAMPT. Transcription Factors: CBFA2T3CLOCK, CREBBP (CBP), EGR3, FOXC1, FOXD3, FOXF1, HEY1, HEY2, HEYL, JUN, MSC, MYF5, NOTCH1, PAX6, PBX1, RBPJ, RBPJL, RUNX1 (AML1), RUNX2, SOX9, TCF15. Other Genes: FJX1, GPSM2, HSPB8, MARK1, RHOV, TEC. |
