地區(qū): | 上海 |
簡(jiǎn)介: | 上海 |
提供商: | SABio |
服務(wù)名稱: | PCR芯片 |
NFkB 信號(hào)通路PCR芯片可用于研究參與NFkB 信號(hào)通路的84個(gè)關(guān)鍵基因的表達(dá)。通過(guò)該芯片,可以確定NFkB 信號(hào)通路在實(shí)驗(yàn)樣本中是被激活,抑制還是沒有變化。芯片涵蓋編碼Rel,NFkB和I kB家族,NFkB-效應(yīng)基因,活化這條通路的細(xì)胞外配體及受體,細(xì)胞內(nèi)轉(zhuǎn)導(dǎo)信號(hào)的激酶和轉(zhuǎn)錄因子。NFkB介導(dǎo)的信號(hào)通路調(diào)控病毒的復(fù)制,自身免疫性疾病,免疫反應(yīng),腫瘤形成和凋亡等過(guò)程。芯片上還包括16個(gè)已經(jīng)被實(shí)驗(yàn)證實(shí)的線粒體能量代謝信號(hào)通路的生物標(biāo)識(shí)物基因。這16個(gè)基因的表達(dá)水平可以通過(guò)公式計(jì)算得出經(jīng)典NFkB通路活性的分?jǐn)?shù)。利用實(shí)時(shí)定量PCR,研究者可以方便并且可信地對(duì)NFkB信號(hào)轉(zhuǎn)導(dǎo)通路相關(guān)的基因進(jìn)行同時(shí)檢測(cè)。 NFκB Pathway: Ligands and Receptors: CD40 (TNFRSF5), CD83, EGFR, FASLG (TNFSF6), IL10, IL1A, IL1B, IL1R1, IL8, LTBR, NOD1 (CARD4), TLR1, TLR2, TLR3, TLR4, TLR6, TLR9, TNF, TNFRSF1A, TNFRSF10A, TNFRSF10B, TNFSF10. Downstream Signaling: BIRC2 (c-IAP2), FADD, IRAK1, IRAK2, IRF1, MYD88, RIPK1, TBK1, TICAM1 (TRIF), TNFAIP3, TRADD, TRAF2, TRAF3, TRAF6. Cytoplasmic Sequestering/Releasing of NFκB: BCL3, CHUK (IKKa), IKBKB, IKBKG, NFKBIA, NFKBIE. Transcription Factors: NFKB1, NFKB2, REL, RELA, RELB. NFκB Responsive Genes: Immune Response: CCL2 (MCP-1), CCL5 (RANTES), CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF),ICAM1, IFNA1, IFNG, IL8, LTA (TNFB), TNF. Apoptosis: AGT, BCL2A1 (BCL-X), BCL2L1, BIRC3 (c-IAP1). Other Factors Involved in NFκB Pathway: Kinases: AKT1, MAP3K1, RAF1. Transcription Factors: ATF1, EGR1, ELK1, FOS, JUN, STAT1. Other Genes: CARD11, CASP1 (ICE), CASP8, CFLAR (CASPER), HMOX1, MALT1, PSIP1, RHOA, TIMP1. Pathway Activity Signature Genes: BIRC3, CCL20, CD83, CXCL2, CXCL3, ICAM1, IL8, IRF1, NFKB1, NFKBIA, NFKBIE, STX11, TIFA, TNF, TNFAIP2, TNFAIP3. |