白血病PCR芯片可用于研究與白血病的發(fā)生、分類和治療反應(yīng)有關(guān)的84個(gè)關(guān)鍵基因的表達(dá)。白血病涵蓋了一系列稱為血液腫瘤的疾病,但主要是指血癌或白細(xì)胞異常增加的骨髓疾病。白血病可以根據(jù)其發(fā)病情況劃分為急性或慢性,會(huì)影響正常的血液細(xì)胞功能和分子生物學(xué)通路,包括炎癥和免疫反應(yīng)、JAK-STAT信號(hào)通路、淋巴細(xì)胞和骨髓細(xì)胞的發(fā)育和分化。芯片包含這些通路的相關(guān)基因,常見(jiàn)的白血病治療靶基因,以及通過(guò)分子生物學(xué)分析與高通量芯片篩查檢測(cè)白血病樣本得到的表達(dá)差異基因和啟動(dòng)子甲基化的差異基因。通過(guò)實(shí)時(shí)定量PCR的方法,研究者即能夠利用該芯片簡(jiǎn)單可靠地同時(shí)檢測(cè)白血病的發(fā)生和發(fā)展相關(guān)的基因表達(dá)。 Differentially Methylated Promoters: APAF1, CADM1, CDH1, CDH13, CDKN1A, CDKN1B, CDKN1C, CDKN2B, DAPK1, DKK3, DLC1, EGR3, HIC1, JUNB, MEIS1, MLH1, NPM1, PTEN, SFRP2, SFRP4, SFRP5, SOCS1, TP53, WIF1. BCR-ABL Pathway: ABL1, BCR, GRB2, JAK2, SHC1, STAT3, STAT5A, STAT5B. JAK-STAT Signaling: AKT1, CSF3, IFNA1, IL4, IL6, JAK2, STAT1, STAT3, STAT5A, STAT5B, SOCS1. Hemopoiesis: BCL2, CSF3, CTNNB1, IL4, JAK2, LMO2, PML, RUNX1, STAT5A, STAT5B, TAL1, TP53. Myeloid Cell Differentiation: CTNNB1, FOXO3, IL4, JUN, LMO2, RUNX1, SPI1, STAT5A, STAT5B, TAL1. Immune System Development: BCL2, CSF3, CTNNB1, IL4, JAK2, LMO2, MLH1, PML, RUNX1, STAT5A, STAT5B, TAL1, TLX1, TP53. Lymphocyte Proliferation/Differentiation: BCL2, CSF3, CTNNB1, IL4, IL6, STAT5A, STAT5B. Cytokines & Chemokines: CCL3, CSF3, CXCL10, IFNA1, IL1R1, IL4, IL6, IL8, IL10, IL12A. Malignancy: BAALC, BMPR1B, BTG3, CD34, CDC42EP3, CTGF, GAS2L3, MN1, NR4A3, RGS12. Leukemia Therapeutic Targets: AKT1, ALOX5, BCL2, CTNNB1, FGR, FOXO3, HCK, HDAC1, HSP90AA1, IFNA1, JAK2, MCL1, MERTK, MTOR, PML, PRKCB, RAC2, SMO. Leukemia Stem Cell Transcriptional Regulators: CEBPB, GATA1, IKZF3, JUN, LMO1, LMO2, LYL1, MEIS1, NFKB1, RUNX1, RUNX2, RUNX3, SPI1, STAT1, STAT3, STAT5A, TAL1, TLX1, TLX3. |