細胞能動性PCR芯片 Cell Motility PCR Array

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細胞能動性PCR芯片 Cell Motility PCR Array

細胞能動性PCR芯片 Cell Motility PCR Array
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地區(qū):上海
簡介:上海
提供商:SABio
服務(wù)名稱:PCR芯片
細胞能動性PCR芯片可用于分析參與細胞運動的84個關(guān)鍵基因的表達。許多細胞依賴細胞運動開展生物過程,如發(fā)育、感染和損傷反應(yīng)。生物刺激如生長因子的釋放,會誘導(dǎo)特定的細胞遷移,從而使激活肌動蛋白細胞骨架。肌動蛋白細胞骨架受Rho GTPase家庭調(diào)控,能介導(dǎo)細胞的運動,或影響成熟細胞發(fā)育過程,如軸突。細胞能通過粘附蛋白運動,從血管內(nèi)皮的間質(zhì)白細胞的滾動遷移,到沿著細胞外基質(zhì)的成纖維細胞的運動,都是通過黏附蛋白發(fā)生的。這一芯片包含了生長因子和受體趨化的重要受體基因,參與Rho家族信號通路的粘附分子基因,以及編碼各種細胞組件的基因。通過實時定量PCR的方法,研究者即能夠利用該芯片簡單可靠地同時檢測細胞能動性相關(guān)基因的表達。
Chemotaxis: FGF2, ITGB2, MAPK1 (ERK2), MYH10, MYH9, PLAUR (uPAR), PLD1, PRKCA, RAC2, TGFB1, VEGFA, WASF2, WIPF1. Receptors: EGFR, IGF1R, ITGA4, ITGB1, ITGB2, ITGB3, MET, PLAUR (uPAR), RHO. Growth Factors: CSF1 (MCSF), EGF, FGF2, HGF, IGF1, TGFB1, VEGFA. Rho Family GTPases: Rho Signaling: ACTR2, ACTR3, ARHGDIA, LIMK1, MSN, MYL9, MYLK, PLCG1, PLD1, PRKCA, PTEN, PTPN1, RHO, RHOA, RHOB, RHOC, RND3, ROCK1, VIM. Rac Signaling: ACTR2, ACTR3, BAIAP2, CFL1, CRK, PAK1, PAK4, PLD1, PRKCA, RAC1, RAC2, STAT3, WASF1, WASF2, WASL. Cdc42 Signaling: ACTR2, ACTR3, CDC42, PFN1, WASF1, WASF2, WASL. Adhesion: Cell-Cell Adhesion: DPP4, EGFR, EZR, ITGA4, ITGB1, ITGB2, MSN, MYH9, ROCK1, TGFB1. Cell-Matrix Adhesion: ACTN1, ACTN3, CSF1 (MCSF), ILK, ITGB1, ITGB2, ITGB3, MMP14, PTEN, PTK2B, PXN, RASA1, RHOA. Focal Adhesion: ACTN1, ACTN3, ARHGEF7, BCAR1, CAPN1, CAPN2, CAV1, ENAH, ILK, ITGB1, MYL9, PTK2, PTK2B, PXN, TLN1, VASP, VCL. Leukocyte Adhesion & Rolling: EZR, ITGA4, ITGB1, ITGB2, MSN, ROCK1. Integrin-Mediated Signaling: BCAR1, ILK, ITGA4, ITGB1, ITGB2, ITGB3, MYH9, PTK2. Cellular Projections: Filopodia: BAIAP2, CDC42, DIAPH1, EGFR, ENAH, EZR, MSN, RDX, SVIL, VASP. Lammelipodia: CTTN, DPP4, EGFR, ENAH, FAP, PIK3CA (p110a), PLD1, PTK2, PXN, RDX, SVIL, VASP, VCL, WASF1, WASF2, WASL. Stress Fibers: ACTN4, DIAPH1, MYH10, MYH9, RHOA, RHOB, RHOC. Membrane Blebs: ACTN1, ACTN3, ACTN4, EZR, MYH10, MYH9, MYLK, RND3, ROCK1. Invasive Projections: ACTR2, ACTR3, ARF6, CDC42, CFL1, CTTN, DPP4, EGF, EZR, FAP, MMP14, MMP2 (Gelatinase A), MMP9 (Gelatinase B), MSN, MYH9, PLAUR (uPAR), RAC2, RASA1, SH3PXD2A, SRC, SVIL, TGFB1, VEGFA, WASL, WIPF1. Growth Cones: ARHGEF7, CDC42, CFL1, PTK2B. Membrane Ruffles: ACTR2, ACTR3, ARF6, BAIAP2, BCAR1, CTTN, DIAPH1, EZR, ITGB1, MYH9, RAC1, RAC2, RDX, RHOA, TLN1, WASF2. Cell Polarity: CDC42, CFL1, EZR, IGF1R, ILK, MYH9. Proteolysis: AKT1, CAPN1, CAPN2, DPP4, FAP, HGF, MMP14, MMP2 (Gelatinase A), MMP9 (Gelatinase B), MYH9, PLAUR (uPAR), TIMP2.


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