成骨PCR基因芯片可用于研究與成骨細胞分化相關的84個基因的表達。該芯片包含與骨骼系統(tǒng)發(fā)育以及骨礦物質代謝有關的基因，包括：生長因子，參與成骨細胞的生成、生長、增殖和分化過程的基因，參與骨骼發(fā)育的細胞外基質分子和細胞粘附分子。利用實時定量PCR，研究者可以方便并且可信地分析與成骨細胞分化有關基因的表達。
Skeletal Development: Cartilage Condensation: BMP1, BMPR1B, COL2A1, SOX9. Ossification: ACVR1, AHSG, BGLAP (Osteocalcin), BMP1, BMP2, BMP3, BMP4, BMP5, BMP6, BMP7, BMPR1A, BMPR1B, BMPR2, CDH11, CHRD, COL1A1, COL2A1, CSF1 (MCSF), CTSK, DLX5, EGFR, FGF2 (bFGF), FGFR2, GDF10 (BMP3B), GLI1, IGF1, IGF1R, IGF2, IHH, MMP2, MMP8, MMP9, NOG, RUNX2, SMAD1, SMAD3, SOX9, SP7, SPP1 (Osteopontin), TGFB1, TGFB2, TGFB3, TNFSF11, TWIST1. Osteoclast Differentiation: BGLAP (Osteocalcin), CSF1 (MCSF), TNF, TNFSF11. Osteoblast Differentiation: ACVR1, BGLAP (Osteocalcin), BMP2, BMP4, BMP6, BMP7, BMPR1A, BMPR1B, BMPR2, CHRD, COL1A1, DLX5, FGF2 (bFGF), FGFR2, GDF10 (BMP3B), GLI1, IGF1, IHH, NOG, RUNX2, SMAD1, SMAD3, SP7, SPP1 (Osteopontin), TWIST1. Other Genes: ALPL, COMP, FGFR1, TGFBR1, TGFBR2. Bone Mineral Metabolism: Bone Mineralization: ACVR1AHSG, BGLAP (Osteocalcin), BMP2, BMP4, BMP6, BMP7, BMPR1A, BMPR1B, BMPR2, FGFR2, IGF1, SMAD3, SOX9, TGFB1, TGFB3, TWIST1. Calcium Ion Binding and Homeostasis: ANXA5, BGLAP (Osteocalcin), CALCR, CDH11, COMP, EGF, FGF2 (bFGF), ITGB1, MMP2, MMP8, TGFB1, VDR. Extracellular Matrix (ECM) Molecules: Collagens: COL10A1, COL14A1, COL15A1, COL1A1, COL1A2, COL2A1, COL3A1, COL5A1. ECM Protease Inhibitors: AHSG, SERPINH1. ECM Proteases: CTSK, MMP10, MMP2, MMP8, MMP9, PHEX. Other Genes: ALPL, BGN, FLT1. Cell Adhesion Molecules: Cell-Cell Adhesion: BMP1, BMPR1B, CDH11, COL14A1, COL2A1, EGFR, ICAM1, IHH, ITGB1, SOX9, TGFB1, TNF, TNFSF11, VCAM1. Cell-Matrix Adhesion: CD36, COL3A1, CSF1 (MCSF), ITGA1, ITGA2, ITGA3, ITGAM, ITGB1, SMAD3. Other Genes: BGLAP (Osteocalcin), COL15A1, COL5A1, COMP, FN1, TNF. Growth Factors: CSF2 (GM-CSF), CSF3 (GCSF), EGF, FGF1, FGF2 (bFGF), GDF10 (BMP3B), IGF1, IGF2, PDGFA, VEGFA, VEGFB. Transcription Factors: GLI1, NFKB1, RUNX2, SMAD1, SMAD2, SMAD3, SMAD4, SMAD5, SOX9, TWIST1. |
