地區(qū): | 上海 |
簡介: | 上海 |
提供商: | SABio |
服務(wù)名稱: | PCR芯片 |
細(xì)胞壞死PCR芯片可用于研究與細(xì)胞壞死相關(guān)的84個(gè)關(guān)鍵基因的表達(dá)。細(xì)胞壞死(也被稱為壞死性凋亡,編程性壞死,脹亡或三型細(xì)胞死亡)最近被證明是一個(gè)受特定調(diào)控的細(xì)胞過程。通過絲氨酸/酪氨酸激酶RIPK1活化的死亡受體包括(FAS,TNRSF1A以及TNFRSF10A)。RIPK1 和RIPK3以及活化的PARP1相互作用導(dǎo)致一些線粒體相關(guān)的效應(yīng)沒,比如:活性氧種類(ROS)的增加,細(xì)胞溶質(zhì)鈣的增加以及ATP的缺失。這張芯片包括程序性壞死相關(guān)的基因,死亡受體相信號(hào),ROS的產(chǎn)生或線粒體活性。細(xì)胞壞死和調(diào)亡可以激活相同的死亡受體,因此,芯片上還包括細(xì)胞死亡程序的下游效應(yīng)分子,從而可以發(fā)現(xiàn)細(xì)胞壞死的新的分子機(jī)制。利用實(shí)時(shí)定量PCR,研究者可以方便并且可信地對(duì)與細(xì)胞程序性壞死相關(guān)的基因進(jìn)行同時(shí)檢測。 Necrosis: Regulation of Necrosis: FAS (TNFRSF6), FASLG (TNFSF6), RIPK1, SLC25A4, TNF. Required for TNF-Induced Necrosis: ATP6V1G2, BMF, C1orf159, CCDC103, COMMD4, CYLD, DEFB1, DENND4A, DPYSL4, EIF5B, FOXI1, GALNT5, GRB2, HSPBAP1, JPH3, KCNIP1, MAG, OR10J3, PARP2, PVR, RAB25, S100A7A, SPATA2, SYCP2, TMEM57, TXNL4B. Other Necrosis Genes: CD40 (TNFRSF5), FADD, MGEA5, RIPK1, RIPK3, PARP1 (ADPRT1), PPIA, SP1, TMEM123. Potential Necrosis Genes: RIPK1 Interactors: FUS, NFKB1, RIPK2, RIPK3. RIPK3 Interactors: GLUD1, GLUL, PYGL, RIPK1. PARP1 Interactors: CAPN1, CAPN2, CAPN3, CAPN5, CAPN6, CAPN7, CAPNS1, PPID. Death Domain Receptors: FAS (TNFRSF6), TNFRSF1A (TNFR1), TNFRSF10A (TRAIL-R). Death Receptor Signaling: BID, BIRC3 (c-IAP1), CASP8AP2, CYLD, EDA2R, FADD, FAF1, FEM1B, IKBKG, LRDD (PIDD), MADD, MYD88, NGF, NGFR, NGFRAP1, PARP1 (ADPRT1), RIPK1, TNFRSF14, TNFRSF17, TNFRSF1B, TNFRSF25 (DR3), TNFRSF4 (OX40), TNFRSF8, TNFSF15, TRADD, TRAF2. Apoptosis: Pro-Apoptosis: AIFM1 (PDCD8), BAX, BID, BMF, BNIP3L, CASP8AP2, FADD, FAF1, FAS (TNFRSF6), FASLG (TNFSF6), FEM1B, IKBKG, LRDD (PIDD), MADD, NGFR, NGFRAP1, RIPK1, RIPK2, RIPK3, TNF, TNFRSF10A (TRAIL-R), TNFRSF25 (DR3), TNFRSF8, TNFSF10 (TRAIL), TRADD, TRAF2. Anti-Apoptosis: BIRC3 (c-IAP1), BNIP3L, FAS (TNFRSF6), MYD88, NFKB1, NGF, NGFR, RIPK1, RIPK2, TNF, TNFRSF1A, TNFRSF1B, TNFRSF4 (OX40), TRAF2. Required for TNF-Induced Apoptosis: ATP6V1G2, CYLD, SPATA2, SYCP2. Reactive Oxygen Species (ROS) Activity: AIFM1 (PDCD8), CYBB, GLUD1, NOX1, NOX4. Mitochondrial Genes: AIFM1 (PDCD8), BAX, BID, BNIP3L, CYBB, GLUD1, GLUL, PPID, RIPK1, SLC25A4 |