地區(qū): | 上海 |
簡(jiǎn)介: | 上海 |
提供商: | SABio |
服務(wù)名稱: | pcr芯片 |
表觀遺傳染色體修飾酶PCR芯片可用于研究與基因組DNA和調(diào)節(jié)染色體可塑性組蛋白修飾有關(guān)的84個(gè)關(guān)鍵基因的表達(dá),這些基因包括已知的或推測(cè)的。維持甲基化的DNA甲基轉(zhuǎn)移酶,負(fù)責(zé)CpG雙核苷脫甲基酶,催化組蛋白乙酰化、甲基化、磷酸化、泛素化的酶以及去乙酰酶和去甲基化酶都在此芯片上。該芯片還分析編碼SET編碼域的蛋白,他們都含有一個(gè)共同的編碼域,用以闡述對(duì)一些家族成員的組蛋白甲基轉(zhuǎn)移酶活性。在干細(xì)胞的發(fā)育晚期成為分化細(xì)胞,基因表達(dá)改變的發(fā)生可以用來調(diào)控染色體動(dòng)力學(xué)。腫瘤細(xì)胞也展現(xiàn)出相對(duì)于正常細(xì)胞不同的基因表達(dá)譜,這表明在腫瘤生成中染色質(zhì)修飾和重構(gòu)的作用。通過實(shí)時(shí)定量PCR的方法,研究者即能夠利用該芯片簡(jiǎn)單可靠地同時(shí)檢測(cè)與表觀遺傳染色體修飾酶有關(guān)的基因表達(dá)。 DNA Methyltransferases: DNMT1, DNMT3A, DNMT3B. Histone Acetyltransferase: ATF2, CDYL, CIITA, CSRP2BP, ESCO1, ESCO2, HAT1, KAT2A (GCN5L2), KAT2B (PCAF), KAT5 (HTATIP), KAT8, KAT7, KAT6A, KAT6B, NCOA1, NCOA3, NCOA6. Histone Methyltransferases: CARM1 (PRMT4), DOT1L, EHMT2, MLL, MLL3, PRMT1, PRMT2, PRMT3, PRMT5, PRMT6, PRMT7, PRMT8, SETDB2, SMYD3, SUV39H1. SET Domain Proteins (Histone Methyltransfesase Activity): ASH1L, MLL3, MLL5, NSD1, SETD1A, SETD1B, SETD2, SETD3, SETD4, SETD5, SETD6, SETD7, SETD8, SETDB1, SUV39H1, SUV420H1, WHSC1. Histone Phosphorylation: AURKA, AURKB, AURKC, NEK6, PAK1, RPS6KA3, RPS6KA5. Histone Ubiquitination: DZIP3, MYSM1, RNF2, RNF20, UBE2A, UBE2B, USP16, USP21, USP22. DNA / Histone Demethylases: KDM1A, KDM5B, KDM5C, KDM4A, KDM4C, KDM6B, MBD2. Histone Deacetylases: HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11.DNA Methyltransferases: DNMT1, DNMT3A, DNMT3B. Histone Acetyltransferase: ATF2, CDYL, CIITA, CSRP2BP, ESCO1, ESCO2, HAT1, KAT2A (GCN5L2), KAT2B (PCAF), KAT5 (HTATIP), KAT8, KAT7, KAT6A, KAT6B, NCOA1, NCOA3, NCOA6. Histone Methyltransferases: CARM1 (PRMT4), DOT1L, EHMT2, MLL, MLL3, PRMT1, PRMT2, PRMT3, PRMT5, PRMT6, PRMT7, PRMT8, SETDB2, SMYD3, SUV39H1. SET Domain Proteins (Histone Methyltransfesase Activity): ASH1L, MLL3, MLL5, NSD1, SETD1A, SETD1B, SETD2, SETD3, SETD4, SETD5, SETD6, SETD7, SETD8, SETDB1, SUV39H1, SUV420H1, WHSC1. Histone Phosphorylation: AURKA, AURKB, AURKC, NEK6, PAK1, RPS6KA3, RPS6KA5. Histone Ubiquitination: DZIP3, MYSM1, RNF2, RNF20, UBE2A, UBE2B, USP16, USP21, USP22. DNA / Histone Demethylases: KDM1A, KDM5B, KDM5C, KDM4A, KDM4C, KDM6B, MBD2. Histone Deacetylases: HDAC1, HDAC2, HDAC3, HDAC4, HDAC5, HDAC6, HDAC7, HDAC8, HDAC9, HDAC10, HDAC11. |