類mTOR信號通路PCR芯片可用于研究mTOR信號通路中的84個關(guān)鍵基因的表達。mTOR是一類絲氨酸/酪氨酸蛋白激酶，整合了來自各方面的信號（如營養(yǎng)，荷爾蒙，生長因子以及細胞壓力等）來調(diào)節(jié)細胞的生長，代謝以及生存。mTOR激酶可以形成兩類蛋白復(fù)合物mTORC1 和mTORC1。 mTORC1調(diào)控多個細胞生物合成過程（蛋白合成細胞周期進程，細胞生長以及增殖）。mTORC2能激活A(yù)KT，對于細胞的增殖，遷移和存活很重要。該芯片中包含mTORC1和mTORC2復(fù)合物中的成員蛋白基因，調(diào)控mTOR反應(yīng)的因子，及受mTOR調(diào)控下游基因。利用實時定量PCR，研究者可以方便并且可信地對與mTOR相關(guān)的基因進行同時檢測。
mTOR Complexes: mTORC1: MLST8, MTOR, RPTOR. mTORC2: MLST8, MAPKAP1 (SIN1), MTOR, RICTOR. mTOR Upstream Regulators: mTORC1 Positive Regulators: AKT1, AKT2, AKT3, HRAS, IGF1, IKBKB, INS, INSR, IRS1, MAPK1, MAPK3, PDPK1, PIK3C3, PIK3CA, PIK3CB, PIK3CD, PIK3CG, PLD1, PLD2, RHEB, RPS6KA1, RPS6KA2, RPS6KA5, RRAGA, RRAGB, RRAGC, RRAGD, TELO2. mTORC2 Positive Regulators: AKT1, AKT2, AKT3, MAPK1, MAPK3, PDPK1, PIK3C3 (VPS34), PIK3CA, PIK3CB, PIK3CD, PIK3CG, RHEB,RPS6KA1, RPS6KA2, RPS6KA5. mTORC1 Negative Regulators: AKT1S1 (PRAS40), CAB39, CAB39L, DDIT4, DDIT4L, DEPTOR, FKBP1A (FKBP12), FKBP8, IGFBP3, PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, PTEN, STK11 (LKB1), STRADB, TP53, TSC1, TSC2, YWHAQ (14-3-3θ). mTORC2 Negative Regulators: CAB39, CAB39L, DDIT4 (REDD1), DDIT4L (REDD2), DEPTOR, PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, STK11 (LKB1), STRADB, TSC1, TSC2. mTOR Downstream Effectors: mTORC1 Positive Regulation: CHUK (IKKa), EIF4B, EIF4E, HIF1A, IKBKB, RPS6, RPS6KB1, RPS6KB2, TP53, VEGFA, VEGFB, VEGFC. mTORC2 Positive Regulation: AKT1, CDC42, GSK3B, HSPA4, ILK, MYO1C, PRKCA, PRKCB, PRKCE, PRKCG, RHOA, RPS6KB1, SGK1. mTORC1 Negative Regulation: EIF4EBP1, EIF4EBP2, PPP2CA, PPP2R2B, PPP2R4, TP53, ULK1, ULK2. Cellular Processes: Amino Acid Response: PIK3C3 (VPS34), RRAGA, RRAGB, RRAGC, RRAGD. Angiogenesis: CHUK (IKKa), DDIT4 (REDD1), DDIT4L (REDD2), HIF1A, IKBKB, VEGFA, VEGFB, VEGFC. Autophagy: PIK3C3 (VPS34), ULK1, ULK2. Cytoskeletal Organization: CDC42, PRKCA, PRKCB, PRKCE, PRKCG, RHOA. Growth Factor Response: HRAS, IGF1, IGFBP3, MAPK1, MAPK3, RPS6KA1, RPS6KA2, RPS6KA5. Energy Stress: PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3. Insulin Signaling: AKT1, AKT2, AKT3, INS, INSR, IRS1, PDPK1, PIK3CA, PIK3CB, PIK3CD, PIK3CG, TP53. Lipid Metabolism: PLD1, PLD2. Translation: EIF4B, EIF4E, EIF4EBP1, EIF4EBP2, PPP2CA, PPP2R2B, PPP2R4, RPS6, RPS6KA1, RPS6KA2, RPS6KA5, RPS6KB1, RPS6KB2. |
