乳腺癌PCR芯片可用于研究與乳腺癌失調、乳腺癌細胞信號轉導，及其他生物過程失調有關的84個關鍵基因的表達。乳腺癌是一種異質性疾病，在分子層面上可以分為正常乳腺癌樣、管腔、HER2像，和基底樣（稱為三陰性）腫瘤。對致癌機制的深入研究表明，某些基因表達水平的變化將影響細胞信號轉導和整個生物通路功能，從而導致癌癥發(fā)生。通過研究這些失調的基因和已知腫瘤分類標志物即可識別乳腺癌的發(fā)生、發(fā)展和轉移。這一芯片包含了腫瘤分類的標志物基因，腫瘤信號轉導相關基因，以及其他相關途徑基因，如血管生成、粘附、蛋白水解、細胞周期和細胞凋亡。通過實時定量PCR的方法，研究者即能夠利用該芯片簡單可靠地同時檢測乳腺癌發(fā)生相關基因的表達。
Tumor Classification Markers: Luminal A-C: ESR1 (ERa), FOXA1, GATA3, KRT8, KRT18, SLC39A6, TFF3, XBP1. HER2-like: ERBB2 (HER2), GRB7. Basal-like / Triple Negative: BIRC5, EGFR, KRT5, NOTCH1. Metastasis to Lung: ID1, MMP2 (Gelatinase A), PTGS2 (COX2). Signal Transduction: Steroid Receptor-Mediated: AR, BRCA1, CCNE1, CTNNB1, ESR1 (ERa), ESR2 (ER?), IGF1, KRT19, PGR, RB1. Hedgehog: BCL2, CCND1, GLI1, SNAI2. Glucocorticoid: IGFBP3, NME1 (NM23A), NR3C1 (GRL). Classical WNT: APC, CCND1, CTNNB1, SFRP1. PI3K/AKT: AKT1, ERBB2 (HER2), IGF1, IGF1R, PTEN. NOTCH: BIRC5, NOTCH1. MAPK: MAPK1 (ERK2), MAPK3 (ERK1), MAPK8 (JNK1), TP73. Epithelial to Mesenchymal Transition: CTNNB1, NOTCH1, SRC, TGFB1, TWIST1. Angiogenesis: CDH13, CTNNB1, EGF, ERBB2 (HER2), ID1, IL6, JUN, NOTCH1, PLAU (uPA), PTEN, SERPINE1 (PAI-1), SLIT2, THBS1, VEGFA. Adhesion: ADAM23, APC, BCL2, CDH1 (E-Cadherin), CDH13, CDKN2A (p16INK4), CSF1 (MCSF), CTNNB1, EGFR, ERBB2 (HER2), PTEN, TGFB1, THBS1. Proteolysis: ADAM23, CST6, CTSD, MMP2 (Gelatinase A), MMP9 (Gelatinase B), PLAU (uPA), PYCARD (ASC). Apoptosis: AKT1, APC, BAD, BCL2, CDH1 (E-CADHERIN), CDH1 (E-CADHERIN)3, CDKN1A (P21CIP1/WAF1), CDKN2A (p16INK4), GSTP1, IGF1, IL6, JUN, MUC1, NME1 (NM23A), RARB, SFN (14-3-3s), SFRP1, TP53, TP73, TWIST1. Cell Cycle: APC, BCL2, CCNA1, CCND1, CCND2, CCNE1, CDK2, CDKN1A (p21CIP1/WAF1), CDKN1C (p57KIP2), CDKN2A (p16INK4), JUN, MKI67, MYC, PTEN, RASSF1, RB1, SFN (14-3-3s), TP53. DNA Damage: APC, ATM, BRCA1, BRCA2, CCND1, CDKN1A (p21CIP1/WAF1), MAPK1 (ERK2), MGMT (AGT), MLH1, SFN (14-3-3s), TP53, TP73. Xenobiotic Transport: ABCB1 (MDR-1), ABCG2 (BCRP). Transcription Factors: AR, CTNNB1, ESR1 (ERa), ESR2 (ER?), FOXA1, GATA3, HIC1, JUN, MYC, NOTCH1, NR3C1 (GRL), PGR, PRDM2 (RIZ1), RARB, RB1, TP53, TP73, XBP1. |
