肝癌PCR芯片可用于研究與肝癌發(fā)生和發(fā)展中關(guān)鍵的84個基因的表達。這些基因參與了重要的生物學(xué)過程或者信號通路。對這些基因進行研究可以幫助我們了解肝癌發(fā)生的分子機制。該芯片中包含有肝癌中經(jīng)常發(fā)生表達變化的基因，關(guān)鍵信號通路中的基因，參與上皮細胞間質(zhì)轉(zhuǎn)化的基因，細胞周期，調(diào)亡以及炎癥相關(guān)的基因。利用實時定量PCR，研究者可以方便并且可信地對與肝癌發(fā)生相關(guān)的基因進行同時檢測。
Genes Up-Regulated During HCC: BCL2, BIRC5, CCND1, CFLAR (CASPER), FZD7, IGF2, IRS1, LEF1, MCL1, MET, NFKB1, PIN1, PTK2, RAC1, RHOA, SMAD7, STAT3, TCF4, TGFA, TGFB1, TNFSF10 (TRAIL), XIAP. Genes Down-Regulated During HCC: BAX, BID, CASP8 (FLICE), CDKN1A (p21CIP1/WAF1), CDKN2A (p16INK4), DLC1, FADD, FAS (TNFRSF6), HHIP, IGFBP1, IGFBP3, ITGB1, PTEN, RB1, SMAD4, SOCS1, SOCS3, TGFBR2, TNFRSF10B (DR5). Signal Transduction: Classical WNT: CCND1, CDKN2A (p16INK4), CTNNB1, FZD7, LEF1, MTDH, PIN1, RB1, SFRP2, TCF4. TGF?: SMAD4, SMAD7, TGFB1, TGFBR2. PI3K/AKT: AKT1, IRS1, PTEN. IGF/IGFR: IGF2, IGFBP1, IGFBP3, IRS1. Hippo: YAP1. Ras/Raf/MEK/ERK: CDKN1A (p21CIP1/WAF1), CDKN2A (p16INK4), DLC1, HRAS, NRAS, RAC1, RASSF1, RB1, RHOA, TP53. EGFR: ADAM17 (CD156B), CDH13, EGF, EGFR, TGFA, TGFB1. MET/HGF: MET, HGF. Small GTPase-Mediated: CDH13, CDKN1A (p21CIP1/WAF1), CDKN2A (p16INK4), DAB2IP, DLC1, HRAS, NRAS, RAC1, RASSF1, RB1, RELN, RHOA, TP53. Epithelial to Mesenchymal Transition (EMT): CTNNB1, HGF, LEF1, SMAD4, SMAD7, TGFB1. Cell Cycle: BIRC5, CCND1, CCND2, CDKN1A (p21CIP1/WAF1), CDKN1B (p27KIP1), CDKN2A (p16INK4), E2F1, EP300, GADD45B, IGF2, MYC, PTEN, RASSF1, RUNX3. Apoptosis: BAX, BCL2, BCL2L1 (BCL-X), BID, BIRC2 (c-IAP2), BIRC5, CASP8 (FLICE), CFLAR (CASPER), DLC1, E2F1, EP300, FADD, FAS (TNFRSF6), FHIT, GADD45B, GSTP1, MCL1, MSH2, PYCARD (ASC), RUNX3, SOCS3, TERT, TNFRSF10B (DR5), TNFSF10 (TRAIL), WT1. Immune & Inflammatory Response: CCL5 (RANTES), CXCR4, NFKB1, PTGS2 (COX2), RAC1, TGFB1, TLR4. Adhesion & Proteolysis: ADAM17 (CD156B), AKT1, CDH1 (E-Cadherin), CDH13, CFLAR (CASPER), DLC1, EGFR, EP300, HGF, ITGB1, LEF1, OPCML, PTEN, PYCARD (ASC), RAC1, RELN, RHOA, SMAD7, SOCS1, SOCS3, TGFB1. Angiogenesis: ANGPT2, CASP8 (FLICE), CDH13, CTNNB1, EGF, FLT1, KDR, PDGFRA, PTEN, PTK2, TGFBR2, VEGFA. DNA Damage: CCND1, CDKN1A (p21CIP1/WAF1), MSH2, MSH3, TP53, XIAP. |
