腫瘤耐藥PCR芯片可用于分析與化療生物學反應有關的84個關鍵基因的表達。這一芯片包含了藥物抗性基因(如P-糖蛋白)、I相代謝基因(P450家族)、和II相代謝基因(如各種共價修飾酶),及其他重要的酶編碼基因。此外芯片還包含了與腫瘤相關的基因,如DNA修復酶、細胞周期調控因子及其受體、生長因子、激素和轉錄因子等。通過實時定量PCR的方法,研究者即能夠利用該芯片簡單可靠地同時檢測腫瘤耐藥和代謝相關基因的表達。 Drug Resistance: ABCB1 (MDR1), ABCC1 (MRP1), ABCC2 (MRP2), ABCC3 (MRP3), ABCC5 (MRP5), ABCG2 (BCRP), BAX, BCL2, BCL2L1 (BCL-X), MVP (LRP), RB1, TOP1, TOP2A, TOP2B, TP53. Drug Metabolism: ARNT, BLMH, CLPTM1L (CRR9), CYP1A1, CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A5, DHFR, EPHX1, GSK3A, GSTP1, NAT2, SOD1, SULT1E1 (STE), TPMT, UGCG. DNA Repair: APC, ATM, BRCA1, BRCA2, ERCC3, MSH2, XPA, XPC. Cell Cycle: CCND1, CCNE1, CDK2, CDK4, CDKN1A (p21CIP1/WAF1), CDKN1B (p27KIP1), CDKN2A (p16INK4), CDKN2D (p19INK4D). Growth Factor Receptors: EGFR (ERBB1), ERBB2 (Her2), ERBB3, ERBB4, FGF2, IGF1R, IGF2R, MET. Hormone Receptors: AR, ESR1 (ERa), ESR2 (ER?), PPARA, PPARD, PPARG, RARA, RARB, RARG, RXRA, RXRB. Transcription Factors: AHR, AP1S1, ELK1, FOS, HIF1A, MYC, NFKB1, NFKB2, NFKBIB (TRIP9), NFKBIE, RELB, TNFRSF11A (RANK). |